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Human Mesenchymal Stem Cell Differentiation qPCR Array

英文名:Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

货号:GK094

价格:$250

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ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Differentiation qPCR Array Kit (GQH-MSC) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) biology, pluripotency maintenance, and differentiation. Brief examples of how included genes may be grouped are shown below:     

  • MSC markers: GNL3, BMPR1A, BMPR1B, BMPR2, KIT, NT5E, ENG, ALCAM, ANPEP, CDH2, CD44, PTPRC, THY1, HLA-A
  • Stemness markers: FUT4, PROM1, ABCG2, SOX2, FGF2, INS, LIF
  • Adipogenesis markers: TNFRSF9, ADIPOQ, ADIPOR1/2, CIDEA, CLTCL1, DLK2, FABP4, SLC27As, SLC2A4, LEP, PLIN2
  • Chondrogenesis markers: ACAN, ANXA6, CD151, CHAD, FOXC1/2, MATN1/3/4, MIA, OTOR, SOX5/6/9
  • Myogenic precursor markers: CDH15, CAV1, CD34, FOXK1, MYF5, MYOD1, MYOG, NCAM1, PAX3/7/9
  • Osteoprogenitor markers: ALPP, DCN, MCAM, MEPE, SP7, RUNX2, THPO

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Mesenchymal Stem Cell Differentiation qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCG2

ANXA6

CD44

DCN

FOXC2

ITGA1

MATN4

NGFR

PPARG

SLC27A6

TFRC

β-actin

B

ACAN

BMPR1A

CDH15

DLK1

FOXK1

ITGB1

MCAM

NT5E

PPARGC1A

SLC2A4

THPO

GAPDH

C

ADIPOQ

BMPR1B

CDH2

DLK2

FUT4

ITM2A

MEPE

OTOR

PROM1

SOX2

THY1

LDHA

D

ADIPOR1

BMPR2

CHAD

ENG

GNL3

KIT

MIA

PAX3

PTPRC

SOX5

TNFRSF9

NONO

E

ADIPOR2

BSCL2

CIDEA

FABP4

HLA-A

LEP

MYF5

PAX7

RUNX2

SOX6

UCP1

PPIH

F

ALCAM

CAV1

CLTCL1

FAM20B

IBSP

LIF

MYOD1

PAX9

SLC27A1

SOX9

VCAM1

GDC

G

ALPP

CD151

COL4A1

FGF2

ICAM1

MATN1

MYOG

PDGFRA

SLC27A2

SP7

VIM

PPC

H

ANPEP

CD34

CRTAC1

FOXC1

INS

MATN3

NCAM1

PLIN2

SLC27A4

SUSD2

ZIC1

NTC 

* gene selection may be updated based on new research and development

货号GK094
产地美国
缩写GQH-MSC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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