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人体细胞连接qPCR检测试剂盒

英文名:Human Cell Junctions qPCR Array Kit

货号:GK090

价格:$250

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ScienCell's GeneQuery™ Human Cell Junctions qPCR Array Kit (GQH-JXN) profiles 88 key genes involved in cell junction formation, organization, and maintenance.  This kit surveys cell-cell junctions that are important for cellular communication, paracellular transport, and anchoring.  Brief examples of how included genes may be categorized are shown below:

  • Gap junctions: AP2M1, CLTA, CLTCL1, GJA1, GJB1, PANX1
  • Adherens junctions: ANG, CADM1, CDH2, CDH5, FAT2, NECTIN2
  • Tight junctions: CDX2, CLDN1, CLDN5, JAM2, SNAI1, TJP1 (ZO-1)
  • Desmosomes: DSC1, DSG1, DSP, ITGB4, JUP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

 

GeneQuery™ Human Cell Junctions qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

CADM1

CDH19

CELSR3

CLDN8

CXADR

DSP

GJA5

JAM3

NECTIN3

PECAM1

β-actin

B

ACTA2

CADM3

CDH2

CGN

CLTA

DCHS1

F11R

GJB1

JUP

OCLN

PVR

GAPDH

C

ACTB

CDH1

CDH3

CLDN1

CLTB

DCHS2

FAT1

GJB6

LEF1

PAK1

RHOA

LDHA

D

ACTC1

CDH10

CDH5

CLDN10

CLTC

DNM1

FAT2

ICAM1

MAGI1

PANX1

SNAI1

NONO

E

ACTG1

CDH11

CDX1

CLDN2

CLTCL1

DSC1

FAT3

ILK

MAGI2

PARD3

SP1

PPIH

F

ACTG2

CDH13

CDX2

CLDN4

CSNK1D

DSC2

FAT4

ITGB1

MYLK

PARD6A

SRC

GDC

G

ANG

CDH15

CELSR1

CLDN5

CTNNB1

DSG1

GJA1

ITGB4

NECTIN1

PCDH7

TJP1

PPC

H

AP2M1

CDH16

CELSR2

CLDN7

CTNND1

DSG3

GJA4

JAM2

NECTIN2

PCDH8

WNK4

NTC

 

 * gene selection may be updated based on new research and development

货号GK090
产地美国
缩写GQH-JXN
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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