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人类Ephrin信号qPCR检测试剂盒

英文名:Human Ephrin Signaling qPCR Array Kit

货号:GK089

价格:$250

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ScienCell's GeneQuery™ Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction.  Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation.  Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B.  A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal” and a “reverse signal.”  Brief examples of how included genes may be grouped according to their function are shown below:

  • Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4
  • Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2
  • Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1
  • Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA
  • Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Ephrin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTB

AP2S1

DNM1

EFNB3

EPHB2

GRIA1

LIMK1

MYL6

PIK3CG

RAP1A

TF

β-actin

B

ACTG1

APH1A

EFNA1

EPHA1

EPHB3

GRIN1

LYN

MYL9

PIK3R1

RASA1

TIAM1

GAPDH

C

ACTN1

CAV1

EFNA2

EPHA2

EPHB4

GRIN2B

MAP2K1

NCK1

PSEN2

RGS3

TLN1

LDHA

D

ADAM10

CDC42

EFNA3

EPHA3

FN1

HRAS

MAPK1

NCK2

PSENEN

RHOA

VAV2

NONO

E

AP2A1

CFL1

EFNA4

EPHA4

FYN

ITGA5

MMP2

NCSTN

PTK2

ROCK1

VAV3

PPIH

F

AP2A2

CLTA

EFNA5

EPHA5

GNAI1

ITGAV

MMP9

PAK1

PXN

SDC2

VCL

GDC

G

AP2B1

CLTB

EFNB1

EPHA7

GRB2

ITGB1

MYH10

PAK2

RAC1

SRC

VEGFA

PPC

H

AP2M1

CLTC

EFNB2

EPHB1

GRB7

KDR

MYH9

PIK3CA

RAF1

SYNJ1

WASL

NTC

 

 * gene selection may be updated based on new research and development

货号GK089
产地美国
缩写GQH-EPH
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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