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人类染色质组织和重塑qPCR检测试剂盒

英文名:Human Chromatin Organization and Remodeling qPCR Array Kit

货号:GK087

价格:$250

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ScienCell's GeneQuery™ Human Chromatin Organization and Remodeling qPCR Array Kit (GQH-CHM) profiles 88 key genes involved in regulating chromatin structure.  Chromatin compacts DNA, allowing mitosis while preventing DNA damage and controlling gene expression.  It is a complex macromolecule that is only found in eukaryotes consisting of RNA, DNA, and protein.  The basic proteins of chromatin are histones and histone modifications contribute to altering chromatin packing and structure.  Brief examples of how included genes may be grouped according to their function are shown below:

  • Chromatin remodeling: ATRX, CBX2, CREBBP, EP300, MECOM
  • Chromatin organization: HIST1H3A, HIST1H3J, HIST2H3A, HIST2H3D, HIST3H3
  • Histone acetylation/deacetylation: ELP3, HDAC1, HDAC4, JADE2, KAT6A
  • Histone methylation/demethylation: DNMT3A, DOT1L, EZH2, JMJD6, KDM6A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Chromatin Organization and Remodeling qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACKR1

CCL4

CSF2

EPO

GYPB

ICAM1

IL17A

IL6

NLRP3

SLC4A1

TLR4

β-actin

B

ALB

CCL5

CSF3

F3

GYPC

IFNAR1

IL18

IRF1

NOS2

TFRC

TLR9

GAPDH

C

ANGPT1

CD36

CTLA4

FASLG

HAVCR2

IFNG

IL1A

ITGAL

ORC1

TGFB1

TNF

LDHA

D

ANGPT2

CD40LG

CXCL10

FCGR2A

HBA2

IFNGR1

IL1B

LTA

PECAM1

TGFB2

TNFRSF1A

NONO

E

C5AR1

CD55

CXCL8

FCGR2B

HLA-B

IL10

IL1RN

MIF

PF4

THBD

TNFRSF1B

PPIH

F

CCL11

CD69

CXCL9

FOXP3

HMOX1

IL12A

IL2

MMP9

PROCR

THBS1

VCAM1

GDC

G

CCL2

CD81

CXCR3

G6PD

HP

IL12B

IL4

MYD88

SELE

TIRAP

VEGFA

PPC

H

CCL3

CR1

DHFR

GYPA

HRH1

IL13

IL5

NCR3

SELP

TLR2

VWF

NTC

 

 * gene selection may be updated based on new research and development

货号GK087
产地美国
缩写GQH-CHM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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