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人体轴突导向qPCR检测试剂盒

英文名:Human Axon Guidance qPCR Array kit

货号:GK084

价格:$250

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ScienCell's GeneQuery™ Human Axon Guidance qPCR Array (GQH-AXN) profiles 88 key genes involved in neural pathfinding.  During neural development, neurons send projections otherwise known as axons to reach targets and transmit electric signals.  Axons follow specific paths and the underlying pathfinding mechanism is an area of growing research.  Below are brief examples of how included genes may be grouped according to their signaling pathways or protein classes:

  • Ephrins: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1
  • Semaphorins: SEMA3A, SEMA4D, SEMA5A, SEMA6B, SEMA7A
  • WNT signaling: WNT4, WNT5A, SHH, SMO, FZD3
  • Netrin signaling: NTN1, NTN4, NTNG1, NTNG2, DCC, UNC5A
  • Cell adhesion molecules: CAMK2A, CAMK2B, CAMK2D, CAMK2G

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Axon Guidance qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABL1

CAMK2B

EFNA1

EPHA1

EPHB3

NRP1

RAC1

SEMA3D

SEMA4F

SLIT2

TRPC6

β-actin

B

ABLIM3

CAMK2D

EFNA2

EPHA2

EPHB4

NRP2

RAF1

SEMA3E

SEMA4G

SLIT3

UNC5A

GAPDH

C

ARHGEF12

CAMK2G

EFNA3

EPHA3

F2RL2

NTN1

ROBO1

SEMA3F

SEMA5A

SMO

UNC5B

LDHA

D

BMP7

CFL1

EFNA4

EPHA4

FES

NTN4

ROBO2

SEMA3G

SEMA6B

TRPC1

UNC5C

NONO

E

BMPR1B

CFL2

EFNA5

EPHA5

FYN

NTNG1

ROCK1

SEMA4A

SEMA6D

TRPC1

UNC5D

PPIH

F

BMPR2

CXCR4

EFNB1

EPHA7

FZD3

NTNG2

RYK

SEMA4B

SEMA7A

TRPC3

WNT4

GDC

G

BOC

DCC

EFNB2

EPHB1

NCK1

PAK2

SEMA3A

SEMA4C

SHH

TRPC4

WNT5A

PPC

H

CAMK2A

DPYSL2

EFNB3

EPHB2

NFATC2

PAK4

SEMA3C

SEMA4D

SLIT1

TRPC5

WNT5B

NTC

 

 * gene selection may be updated based on new research and development

货号GK084
产地美国
缩写GQH-AXN
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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