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人成骨分化qPCR检测试剂盒

英文名:GeneQuery™ Human Osteogenic Differentiation qPCR Array Kit

货号:GK080

价格:$250

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ScienCell's GeneQuery™ Human Osteogenic Differentiation qPCR Array (GQH-OST) facilitates gene expression profiling of 88 key genes involved in the differentiation of mesenchymal stem cells into osteocytes, osteoblasts, and osteoclasts.  Osteocytes are most commonly found in mature bone tissue and capable of signal transmission over long distances.  Osteoblasts and osteoclasts are bone cells that synthesize and break down bone, respectively.  Brief examples of how included genes may be grouped according to their function are shown below:

  • Osteocyte marker: BGN, FGF23, PDPN, HYOU1, SOST
  • Osteoblast marker: GNL3, CD44, OMD, MME, SCUBE3
  • Osteoclast marker: CA2, CALCR, CTSK, MMP9, TNFRSF11A
  • RUNX2 suppressor: GLI3, HES1, STAT1, TWIST1, HAND2
  • BMP signaling: BMP2, BMP4, SMAD4, SMAD5, SMAD9

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Osteogenic Differentiation qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ALCAM

CA2

CSNK2A1

FGF2

FZD9

HYOU1

MAF

NKX3-2

RBPJ

SMO

TCF7

β-actin

B

ALPL

CALCR

CTNNB1

FGF23

GLI2

IBSP

MCAM

NOTCH1

RUNX2

SOST

TGFB1

GAPDH

C

ATF4

CAPG

CTSK

FGFR1

GLI3

IGF1R

MEPE

NOTCH2

SATB2

SOX9

TNFRSF11A

LDHA

D

BCAP31

CD44

DCN

FGFR2

GNL3

IGFBP3

MME

OMD

SCUBE3

SP7

TNFSF11

NONO

E

BGN

CD59

DMP1

FGFR3

HAND2

ITGB1

MMP14

PCOLCE

SMAD1

SPARC

TPO

PPIH

F

BMP2

COL1A1

DSTN

FN1

HES1

LEF1

MMP9

PDPN

SMAD4

SPP1

TWIST1

GDC

G

BMP4

COL1A2

ENG

FOSB

HEY1

LRP5

MSX2

PHEX

SMAD5

STAT1

TXLNG

PPC

H

BMPR1A

COL2A1

FGF18

FOSL1

HIVEP3

LRP6

NFATC1

PRKCD

SMAD9

TAZ

WNT5A

NTC

 * gene selection may be updated based on new research and development

货号GK080
产地美国
缩写GQH-OST
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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