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人类软骨细胞生物学qPCR检测试剂盒

英文名:Human Chondrocyte Biology qPCR Array Kit

货号:GK079

价格:$250

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ScienCell's GeneQuery™ Human Chondrocyte Biology qPCR Array Kit (GQH-CHN) facilitates gene expression profiling of 88 key genes in chondrocyte function.  Chondrocytes are embedded in the cartilage matrix and are responsible for producing and maintaining it.  Chondrocytes originate in the mesenchyme and are hypertrophic when terminally differentiated during ossification.  Brief examples of how included genes may be grouped according to their function in chondrocyte biology are shown below:

  • Chondrogenesis: COL2A1, ACAN, SOX9, MIA, PTCH1
  • ECM interaction: RHOA, ITGAV, CD44, PTK2, ITGB1
  • Mechanotransduction: NFATC4, TRPV4, CREB1, MAPK1, PRKCE
  • Remodeling and repair: BMP2, HIF1A, MMP9, WNT9A, ICAM1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Chondrocyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACAN

CACNA1C

COL2A1

FGFR3

IGF1R

ITGA6

MAPK3

NFATC4

PTGER4

RUNX2

SOX9

β-actin

B

ALPL

CACNA1D

COL4A1

FLNA

IHH

ITGA7

MATN1

NKX3-2

PTH1R

SCNN1A

SP7

GAPDH

C

ANXA5

CD151

COL4A2

FLNB

ILK

ITGAV

MATN3

OTOR

PTHLH

SCNN1B

SPARC

LDHA

D

ANXA6

CD44

CREB1

FOXC1

ITGA1

ITGB1

MATN4

PRG4

PTK2

SCNN1G

TGFB3

NONO

E

AQP1

CD47

CRTAC1

FOXC2

ITGA2

ITGB5

MIA

PRKCA

PXN

SMAD2

TNC

PPIH

F

BMP2

CHAD

CTSB

HIF1A

ITGA3

KCNA4

MMP13

PRKCB

RAC1

SMAD3

TRPV4

GDC

G

BMP4

CILP

DDR2

IBSP

ITGA4

KCNA6

MMP9

PRKCE

RARG

SOX5

VEGFA

PPC

H

CACNA1A

COL11A2

EPYC

ICAM1

ITGA5

MAPK1

NCAM1

PTCH1

RHOA

SOX6

WNT9A

NTC

 * gene selection may be updated based on new research and development

货号GK079
产地美国
缩写GQH-CHN
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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