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人类小胶质细胞Sensome qPCR检测试剂盒

英文名:Human Microglial Sensome qPCR Array Kit

货号:GK073

价格:$250

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ScienCell's GeneQuery™ Human Microglial Sensome qPCR Array (GQH-MGS) facilitates gene expression profiling of 88 key genes involved in how microglia perceive their environment.  Microglia are considered to be “the resident macrophages of the CNS,” so how they sense their environment is integral to maintaining homeostatic functions in the brain during pathogen invasion, injury, or degeneration.  The genes included in this particular array focus on genes with unique differential expression in aging.  Examples of how included genes may be grouped according to their function are shown below:

  • Classical priming: PTGS2, TNF, CXCL10
  • Alternative priming: SPP1, MARCO, CXCR4, CLEC7A, CD163
  • Chemokine receptors: PTAFR, CMKLR1, GPR77, C5AR1, CCR5
  • Purinergic receptors: TMEM173, P2RY1, P2RY12, GPR34, ADORA3
  • Aging: TREM2, TLR8, SCARA3, IFITM3, AGER

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Microglial Sensome qPCR Array Plate Layout* (8 controls in Bold and Italic) 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADORA3

CCL8

CD22

CD86

CXCR2

FCGR2B

HAVCR2

ITGB2

P2RX5

SELPLG

TLR2

β-actin

B

AGER

CCR1

CD33

CLEC5A

CXCR4

FCGR3A

IFITM1

LAIR1

P2RY1

SIGLEC1

TLR7

GAPDH

C

ALOX15

CCR2

CD37

CLEC7A

CYSLTR1

FCRL1

IFITM2

LGALS9

P2RY12

SIGLEC5

TLR8

LDHA

D

ARG1

CCR5

CD48

CMKLR1

ECSCR

FPR2

IFITM3

LTF

P2RY13

SLC2A5

TMEM173

NONO

E

C3AR1

CCRL2

CD52

CMTM7

F13A1

GAS7

IFITM5

LY86

PTAFR

SLC7A7

TNF

PPIH

F

C5AR1

CD14

CD68

CSF1R

FCER1A

GPR183

IL10RA

MARCO

PTGS2

SLCO2B1

TNFRSF1B

GDC

G

C5AR2

CD163

CD74

CX3CR1

FCER1G

GPR34

IL1RN

NRG1

PTPRC

SPP1

TREM2

PPC

H

CCL20

CD180

CD84

CXCL10

FCGR1A

GPR84

ITGAM

OLR1

SCARA3

TGFBR1

TYROBP

NTC

 * gene selection may be updated based on new research and development

货号GK073
产地美国
缩写GQH-MGS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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