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人骨质疏松症qPCR检测试剂盒

英文名:Human Osteoporosis qPCR Array Kit

货号:GK070

价格:$250

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ScienCell's GeneQuery™ Human Osteoporosis qPCR Array (GQH-POR) facilitates gene expression profiling of 88 genes involved in the establishment and progression of osteoporosis.  Osteoporosis is a bone disease in which bone loss, lack of bone growth, or both result in weak and brittle bones.  Complications of osteoporosis include broken bones and fractures.  While osteoporosis affects everyone, older women are at higher risk of developing the disease.  Brief examples of how included genes may be grouped according to their function are shown below:

  • Bone remodeling: ALOX15, COL1A1, COL1A2, IBSP, SP7
  • Calcium regulation: ALB, CALCR, PTH, CYP19A1, CALB1
  • Osteoblast differentiation: ALPL, RUNX2, TNFSF11, TNFRSF11A
  • Cytokines and growth factors: FGF23, BMP2, IGF1, TGFB1, LRP5
  • Susceptibility markers: AR, CASR, ESR2, CTSK, PDLIM4, VDR

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Osteoporosis qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY10

BMP2

COL1A1

CYP27B1

FOS

IGF2

ITGA1

NFATC1

PTH

SOST

TNFRSF11B

β-actin

B

ADIPOQ

BMP7

COL1A2

DKK1

GC

IGFBP2

JUN

NOG

PTH1R

SP7

TNFSF11

GAPDH

C

ALB

CA2

CRTAP

ESR1

GH1

IGFBP4

LEP

NR3C1

PTHLH

SPARC

TWIST1

LDHA

D

ALOX15

CALB1

CSF1

ESR2

GHR

IL1A

LRP5

P3H1

PTK2B

SPP1

UGT2B17

NONO

E

ALPL

CALCA

CTNNB1

FDPS

HMGCR

IL1B

LTBP2

PDLIM4

RUNX2

TGFB1

VDR

PPIH

F

APOE

CALCR

CTSK

FGF23

HSD11B1

IL1RN

MMP14

PLOD2

SFRP1

TIMP2

VEGFA

GDC

G

AR

CASR

CYP19A1

FGFR1

IBSP

IL6

MMP2

PLS3

SLC34A1

TNF

WNT1

PPC

H

ARHGEF3

CD36

CYP24A1

FGFR2

IGF1

IL7

MTHFR

PPARG

SLC6A8

TNFRSF11A

WNT3A

NTC

* gene selection may be updated based on new research and development

货号GK070
产地美国
缩写GQH-POR
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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