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人类小胶质细胞生物学qPCR检测试剂盒

英文名:Human Microglia Cell Biology qPCR Array Kit

货号:GK069

价格:$250

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ScienCell's GeneQuery™ Human Microglia Cell Biology qPCR Array (GQH-MGL) facilitates gene expression profiling of 88 key genes involved in microglial regulation and function.  Microglia are considered to be the main cells of defense in the central nervous system (CNS).  Though often touted as “the resident macrophages of the CNS,” microglia distinguish themselves from macrophages in regulation and through specialized roles.  Examples of how included genes may be grouped according to their function are shown below:

  • Classical priming: BIRC3, IFNG, IL12A, CXCL10, TYMP
  • Alternative priming: GAS7, IGF1, CXCL2, SPP1, MRC1
  • Microglia markers: PTPRC, ITGAX, AIF1, CD40, EDA
  • Sensome: GPR34, CXCR2, ADORA3, LTF, SIGLECH
  • Receptors: MARCO, MSR1, P2RX4, SIRPA, CSF1R

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Microglia Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADORA3

CD40

CX3CR1

F13A1

HLA-DQB1

IRF1

MARCO

NOS2

PTGES

SLCO2B1

TMEM8C

β-actin

B

AIF1

CD47

CXCL10

FGL2

HLA-DRA

IRF8

MMP12

P2RX4

PTGS2

SPI1

TNF

GAPDH

C

BIRC3

CD68

CXCL2

GAS7

HLA-DRB1

ITGAM

MRC1

P2RX7

PTPRC

SPP1

TREM2

LDHA

D

CASP1

CD80

CXCL9

GPR34

IFNG

ITGAX

MSR1

P2RY1

PYCARD

TGFB1

TXNIP

NONO

E

CCL8

CD86

CXCR2

GPR84

IGF1

ITGB5

MYB

P2RY12

RUNX1

TLR2

TYMP

PPIH

F

CD163

CLEC5A

ECSCR

HAVCR2

IL12A

LGALS3

MYD88

P2RY2

SIGLEC5

TLR3

TYROBP

GDC

G

CD200R1

CLEC7A

EDA

HEXB

IL1B

LILRA5

NLRC4

PANX1

SIRPA

TLR4

UPK1B

PPC

H

CD33

CSF1R

EGR2

HLA-DQA1

IL6

LTF

NLRP3

PILRA

SLAMF9

TLR5

XIAP

NTC

 * gene selection may be updated based on new research and development

货号GK069
产地美国
缩写GQH-MGL
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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