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人类平滑肌收缩和疾病qPCR检测试剂盒

英文名:Human Smooth Muscle Contraction and Diseases qPCR Array Kit

货号:GK067

价格:$250

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ScienCell's GeneQuery™ GQH-SMD qPCR Array is designed to profile the gene expression of 88 key genes involved in smooth muscle contraction and diseases that affect smooth muscle function.  Vertebrates have three main types of muscle tissue: cardiac, skeletal, and smooth or non-striated.  Smooth muscle fundamentally differs from the other two types in both regulation and function.  Smooth muscle contraction is involuntary and it is found lining blood vessels, lymphatic vessels, the bladder, and the reproductive, GI, and respiratory tracts.  Smooth muscle malfunctions can lead to a host of disorders such as achalasia (when smooth muscles fail to relax) and Hirschsprung’s (muscle failure in the GI tract due to lack of nerves).  Brief examples of how included genes may be grouped according to relevance are shown below:

  • Smooth muscle contraction: SORBS3, TPM1, MYL9, ITGA1, CALM1, ACTA2
  • Smooth muscle differentiation: CAMK2D, KCNH8, KLF4, MEF2A, SRF
  • Achalasia: RET, NOS1, KIT, FLVCR1, MC2R
  • Hirschsprung’s disease: ECE1, EDN3, EDNRB, GDNF, SOX10

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Smooth Muscle Contraction and Diseases qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AAAS

CALD1

EDN3

HLA-DQA1

KCNN3

MEF2A

MYL6B

NNT

PSPN

SORBS1

TPM1

β-actin

B

ACHE

CALM1

EDNRB

HLA-DQB1

KIDINS220

MEF2B

MYL9

NOS1

PTPN22

SORBS3

TPM2

GAPDH

C

ACTA2

CALM2

ELK1

HMOX2

KIF1BP

MEF2C

MYLK

NOS2

PXDN

SOX10

TPM3

LDHA

D

ACTG2

CAMK2D

FLVCR1

HOXB5

KIT

MEF2D

MYLPF

NPY

PXN

SRF

TPM4

NONO

E

ARTN

CCND2

GDNF

ITGA1

KLF4

MFSD7

MYOCD

NTF3

RASGEF1A

SYP

UCHL1

PPIH

F

ASCL1

CTSD

GFRA1

ITGB5

L1CAM

MYH11

NCAM1

NTRK3

RET

TAC1

VCL

GDC

G

BLVRB

DPYD

GFRA2

KCNH4

LMOD1

MYL12B

NGFR

PAX3

SEMA3C

TLN1

VIPR1

PPC

H

CALB2

ECE1

GUCY1A3

KCNH8

MC2R

MYL6

NKX2-5

PIGV

SEMA3D

TP53

ZEB2

NTC

 * gene selection may be updated based on new research and development

货号GK067
产地美国
缩写GQH-SMD
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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