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人类骨骼肌收缩和肌肉营养不良qPCR检测试剂盒

英文名:Human Skeletal Muscle Contraction and Muscular Dystrophies qPCR Array Kit

货号:GK065

价格:$250

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ScienCell's GeneQuery™ Human Skeletal Muscle Contraction and Muscular Dystrophies qPCR Array (GQH-SKC) profiles 88 key genes involved in the contraction of skeletal muscle.  There are three major muscle types in the human body: skeletal, cardiac, and smooth.  Skeletal muscle is a striated muscle that relaxes and contracts to generate movement and force in the body.  Of the three main muscle types, it is the only one under voluntary control.  Neuromuscular diseases involving skeletal muscle dysfunction are very diverse and range from myopathies to muscular dystrophies.  Below are brief examples of how included genes may be grouped according to their function in skeletal muscle biology:

  • Myogenesis: MYOD1, MYOG, PAX3, UTRN, MYF6, MDTN
  • Contractility: MYH2, SLC2A4, LMNA, DYSF, APT2A1
  • Sarcomere Assembly : NEB, TRIM63, TTN, CAPN3, ACTA1
  • Muscle Atrophy: NOS2, TRIM63, FOXO1, MAPK8, AKT1
  • Muscular Dystrophy Development: POMT2, FKRP, ANO5, FRG1, SGCB

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Skeletal Muscle Contraction and Muscular Dystrophies qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA1

ANO5

CAST

DES

FRG1

LARGE1

MMP9

MYL5

POMGNT2

SEPN1

TNNT2

β-actin

B

ACTA2

ATP2A1

CAV1

DNAJB6

HDAC5

LMNA

MSTN

MYOD1

POMT1

SGCA

TNPO3

GAPDH

C

ACTN3

BCL2

CAV3

DUX4

IGF1

LMOD1

MUSK

MYOG

POMT2

SGCB

TPM1

LDHA

D

ACVR2B

BMP4

COL6A1

DYSF

IGFBP3

MAPK1

MYBPC2

NEB

PPARGC1A

SGCD

TPM3

NONO

E

ADRB2

CAMK2G

COL6A2

EMD

IGFBP5

MAPK14

MYF5

NOS2

PPARGC1B

SLC2A4

TRIM32

PPIH

F

AGRN

CAPN2

COL6A3

FKRP

IKBKB

MAPK8

MYF6

PAX3

PPP3CA

TCAP

TRIM63

GDC

G

AKT1

CAPN3

CRYAB

FKTN

ISPD

MB

MYH2

PAX7

RHOA

TNF

TTN

PPC

H

AKT2

CASP3

DAG1

FOXO1

LAMA2

MEF2C

MYL1

POMGNT1

RPS6KB1

TNNI1

UTRN

NTC


 * gene selection may be updated based on new research and development

 

货号GK065
产地美国
缩写GQH-SKC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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