我的购物车>> 中国区首家总代 专业十六载 打造细胞生物学精品

您当前所在位置:首 页 > 产品展示 > 基因表达分析 > qPCR检测试剂盒 人类骨关节炎和软骨修复qPCR检测试剂盒

人类骨关节炎和软骨修复qPCR检测试剂盒

英文名:Human Osteoarthritis and Cartilage Repair qPCR Array Kit

货号:GK064

价格:$250

产品说明书下载:

产品预订,提交订单信息之后,我们会第一时间与您取得联系!

ScienCell's GeneQuery™ Human Osteoarthritis and Cartilage Repair qPCR Array (GQH-CCR) facilitates gene expression profiling of 88 key genes involved in the establishment and progression of osteoarthritis (OA), focusing on the breakdown of cartilage repair.  OA is characterized by joint swelling, cartilage degradation, and eventually bone breakdown.  While the causes of OA are still unknown, there are genetic components that can determine a person’s susceptibility to the disease.  Brief examples of how included genes may be grouped according to function are shown below:

  • Cartilage homeostasis: FGF18, FGF2, HIF1A, IGF1, SMAD2, TGFB1
  • Inflammation: IL17A, ELF3, IL18, MAPK3, PTGS2, TNF
  • Chondrocyte hypertrophy: CCL19, SIRT1, WNT4, IHH, MMP13
  • Susceptibility genes: SMAD3, ASPN, FRZB, DIO2
  • Markers of moderate or severe OA: TIMP1, COL2A1, FN1, C11orf31, CILP

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Osteoarthritis and Cartilage Repair qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACAN

BMP4

COL1A1

DIO2

FRZB

IL17A

MAPK8

NFKB1

SMAD1

TIMP1

WNT3A

β-actin

B

ADAMTS4

BMP6

COL1A2

DKK1

GADD45B

IL18

MATN3

NFKB2

SMAD2

TLR2

WNT4

GAPDH

C

ADAMTS5

BMP7

COL2A1

ELF3

GDF11

IL1A

MMP1

NOS2

SMAD3

TLR4

WNT5A

LDHA

D

AGER

C11orf31

COL3A1

EPAS1

HIF1A

IL1B

MMP13

NOTCH1

SMAD5

TNC

WNT5B

NONO

E

ALK1

CCL19

COL5A1

FGF18

HMGB1

IL6

MMP2

PTGS2

SMAD9

TNF

WNT6

PPIH

F

ASPN

CCR7

COL5A2

FGF2

HTRA1

MAPK1

MMP3

RUNX2

SOX9

TNFRSF11A

WNT7A

GDC

G

ATP1A1

CILP

CTNNB1

FMOD

IGF1

MAPK14

MMP7

S100B

TGFB1

TNFSF11

WNT8A

PPC

H

BMP2

COL10A1

CXCL8

FN1

IHH

MAPK3

MMP9

SIRT1

TGFBR1

WNT1

WNT9A

NTC

 * gene selection may be updated based on new research and development

货号GK064
产地美国
缩写GQH-OAC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰

相关产品
货号
产品名称
规格
价格
指令
联系电话
+86 4008 125 268
+86 10 63804220
+86 10 63803739
+86 10 63861816
QQ客服