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人细胞周期蛋白和细胞周期蛋白依赖性激酶qPCR检测试剂盒

英文名:Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

货号:GK063

价格:$250

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ScienCell's GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit (GQH-CCN) is designed to facilitate gene expression profiling of 40 selected cyclins and cyclin-dependent kinases (CDKs). CDKs are a family of small enzymes required for cell cycle progression and cell division, by means of phosphorylation of their substrates. CDKs must bind to cyclins to become fully activated. Cyclin/CDK complexes regulate cell cycle checkpoints and are the driving force of cell cycle progression. Brief examples of how included genes may be grouped are shown below:

  • Cyclins: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY
  • CDKs: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

B

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

C

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

D

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

E

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

F

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

G

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

H

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

 

* gene selection may be updated based on new research and development

货号GK063
产地美国
缩写GQH-CCN
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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