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人类脂肪生成qPCR检测试剂盒

英文名:Human Adipogenesis qPCR Array Kit

货号:GK057

价格:$250

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ScienCell's GeneQuery™ Human Adipogenesis qPCR Array (GQH-ADP) facilitates gene expression profiling of 88 key genes involved in adipocyte differentiation.  Adipocytes are also known as fat cells and are the primary component of adipose tissue, the body’s main form of energy storage.  There are two types of adipose tissue in mammals comprised of distinct cell populations: white adipocytes that contain a single lipid droplet and brown adipocytes that contain numerous smaller lipid droplets.  Examples of how included genes may be classified according to function are shown below:

  • Pro-adipogenesis: LPL, SFRP1, AGT, FABP4, EPAS1
  • Anti-adipogenesis: TRIB3, DDIT3, LRP5, SHH, RORA
  • Pro-white adipocytes: CEBPA. KLF15, SREBF1, EGR2, FGF10
  • Pro-brown adipocytes: IRS2, FOXC2, DIO2, CREB1, UCP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Adipogenesis qPCR Array Plate Layout* (8 controls in Bold and Italic) 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADIPOQ

BMP7

DDIT3

FASN

GATA2

IRS2

LPL

NR3C1

RARA

SHH

TRIB3

β-actin

B

ADRB2

CCND1

DIO2

FGF1

GATA3

KLF15

LRP5

NRP1

RB1

SIRT3

TWIST1

GAPDH

C

AGT

CDK4

DKK1

FGF10

GH1

KLF2

MAPK14

PARD3

RBL1

SLC2A4

UCP1

LDHA

D

AGXT

CDKN1A

DLK1

FGF2

HES1

KLF3

NAMPT

PCK2

RETN

SPOCK1

VDR

NONO

E

ANGPT2

CEBPA

E2F1

FOXC2

HMGA1

KLF4

NCOA2

PPARA

RORA

SREBF1

WNT1

PPIH

F

AXIN1

CEBPB

EGR2

FOXO1

HNF1A

LEP

NCOR2

PPARD

RXRA

STAT5B

WNT10B

GDC

G

BMP2

CEBPD

EPAS1

FZD1

IL6

LIF

NR1H3

PPARG

SERPINE1

TAZ

WNT3A

PPC

H

BMP4

CREB1

FABP4

GADD45A

INSR

LMNA

NR2F2

PPARGC1A

SFRP1

TCF7L2

WNT5A

NTC

 * gene selection may be updated based on new research and development

货号GK057
产地美国
缩写GQH-ADP
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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