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人体止血qPCR检测试剂盒

英文名:Human Hemostasis qPCR Array Kit

货号:GK056

价格:$250

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ScienCell's GeneQuery™ Human Hemostasis qPCR Array Kit (GQH-HEM) profiles 88 key genes involved in stopping bleeding.  Upon wounding, hemostasis generally involves vasoconstriction to prevent blood flow to the afflicted area, forming a temporary plug to inhibit bleeding, and then coagulation or blood clotting.  Examples of disorders in hemostasis are hemophilia, thrombosis, and embolisms.  Brief examples of how included genes may be grouped according to their function in hemostasis are shown below:

  • Platelet activation: F2R, FCER1G, FYN, PLA2G4A, PIK3CB
  • Clotting: F8, PF4, PLAT, THBD, COL4A1
  • Coagulation: A2M, F7, PLAUR, C5AR1, C7
  • Hemophilia: F2, F3, VWF, IL10, F5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Hemostasis qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

A2M

APP

F3

FYN

IL3

JAK3

PFN1

PLAUR

PSAP

SOS1

TMSB4X

β-actin

B

ACTN1

C5AR1

F5

GNAQ

IL5

LAT

PIK3CB

PLCG2

PTK2

SPARC

TUBA4A

GAPDH

C

ACTN2

C6

F7

IGF1

IL6

LYN

PIK3CD

PLEK

SELP

SRGN

VAV1

LDHA

D

ADRA2A

C7

F8

IGF2

ITGA2B

MPL

PIK3R1

PLG

SEPP1

TGFB1

VAV2

NONO

E

AKT2

CLU

FCER1G

IL10

ITGB3

MTCH1

PIKFYVE

PRF1

SERPINA1

THBD

VAV3

PPIH

F

AKT3

COL4A1

FIGF

IL2

ITPR1

P2RY12

PLA2G4A

PRKCA

SERPINE1

THBS1

VCL

GDC

G

APBB1IP

F2

FLNA

IL2RA

ITPR2

PECAM1

PLAT

PRKCE

SFTPA1

TIMP1

VEGFA

PPC

H

APOA1

F2R

FN1

IL2RB

JAK2

PF4

PLAU

PRKCQ

SOD1

TLN1

VWF

NTC

  * gene selection may be updated based on new research and development

货号GK056
产地美国
缩写GQH-HEM
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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