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人类丙型肝炎qPCR检测试剂盒

英文名:Human Hepatitis C qPCR Array Kit

货号:GK044

价格:$250

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ScienCell's GeneQuery™ Human Hepatitis C qPCR Array Kit (GQH-HPC) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis C pathogenesis. Hepatitis C  is a liver infectious disease caused by the Hepatitis C virus (HCV). The HCV exposure usually causes persistent liver infection and often develops into chronic liver disease. In response to hepatocyte HCV infection, antiviral immune response cascades are triggered and interferons are produced to modulate the immune system and suppress the spread of HCV. Brief examples of how included genes may be grouped are shown below:     

  • HCV binding factor: LDLR, CD81, OCLN, SCARB1
  • Signaling pathways
    • RIG-1 like receptor: DDX58, MAVS, RAF3, TBK1, IKBKE, IRF3
    • Toll-like receptor: TLR3, TICAM1, RIPK1, IKBIB, IKBKG, CHUK
    • JAK/STAT: IFNAR1, IFNAR2, JAK1, TYK2, STAT1, STAT2, STAT6
  • Genes implicated in HCV infection: IFNA1, IFNA2, GPT, CLDN1, IFNB1, EIF2AK2, CLEC4M, RAF1, IRF3, HM13, GGT1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Hepatitis C qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CLEC4M

EIF3E

IFNA2

IRF3

MAPK14

OAS1

PIK3R1

PPP2R1B

RIPK1

STAT6

β-actin

B

AKT2

DDX58

GGT1

IFNAR1

JAK1

MAPK3

OAS2

PIK3R2

PPP2R2A

RNASEL

TBK1

GAPDH

C

AKT3

EGFR

GPT

IFNAR2

LDLR

MAPK8

OAS3

PIK3R3

PPP2R2B

RXRA

TICAM1

LDHA

D

BAD

EIF2AK1

GRB2

IFNB1

MAPK1

MAPK9

OCLN

PIK3R5

PPP2R2C

SCARB1

TLR3

NONO

E

CD81

EIF2AK2

HM13

IKBKB

MAPK10

MAVS

PDPK1

PPARA

PPP2R2D

SOS1

TNFRSF1A

PPIH

F

CDKN1A

EIF2AK3

IFIT1

IKBKE

MAPK11

NFKB1

PIAS1

PPP2CA

PSME3

SOS2

TRADD

GDC

G

CHUK

EIF2AK4

IFIT1B

IKBKG

MAPK12

NFKBIA

PIK3CB

PPP2CB

RAF1

STAT1

TRAF2

PPC

H

CLDN1

EIF2S1

IFNA1

IRF1

MAPK13

NR1H3

PIK3CG

PPP2R1A

RELA

STAT2

TYK2

NTC

 

* gene selection may be updated based on new research and development

货号GK044
产地美国
缩写GQH-HPC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存 4°C
运输胶冰
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