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人肝细胞癌qPCR检测试剂盒

英文名:Human Hepatocellular Carcinoma qPCR Array Kit

货号:GK042

价格:$250

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ScienCell's GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Kit (GQH-HCC) is designed to facilitate gene expression profiling of 88 key genes involved in hepatocellular carcinoma tumorigenesis. Hepatocellular carcinoma is the most common type of liver cancer. It tends to occur in livers damaged by chronic infections of hepatitis B and/or C, alcohol abuse, type II diabetes and cirrhosis.  Brief examples of how included genes may be grouped are shown below:

  • Chronic hepatitis B virus and hepatitis C virus infection response: AKT1, NFKB1, SOCS3, TGFB1
  • DNA damage response and apoptosis: CASP8, MSH2, BAX, BCL2, BIRC5, FAS, TP53
  • Cell cycling: CCND1, CCND2, CDKN1A, CDKN2A, BIRC5, MYC, PTEN, RASSF1
  • Major signaling pathways involved in HCC tumorigenesis
    • Wnt/β-cateninAXIN1, CCND1, CCND2, CTNNB1, EP300, FZD7, LEF1, RHOA, SFRP2, SMAD4
    • VEGF: VEGFA, AKT1, KDR, PTGS2, PTK2, RAC1, NRAS
    • FGF: AKT1, BAX, NFKB1, RAC1
    • MAPK: EGF, FAS, GADD45B, PDGFRA, TGFB1, TGFBR2, RAC1
    • PI3K: BCL2, FLT1, CREB3L3, IRS1, ITGB1, PTK1, MCL1, PTEN, RELN
  • Other genes with demonstrated implications in HCC development: IGF2R, PDGFRL, MET, GLUL, MTUS1, FGL1, PEG10, MAGEC2, STARD13, FAH, GPC3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CCL5

CLDN10

EP300

GLUL

IGF2BP2

MCL1

NRAS

PTK2

SMAD7

TGFBR2

β-actin

B

ANGPT2

CCND1

CREB3L3

FADD

GPC3

IGF2R

MET

PDGFRA

RAC1

SOCS1

TNFRSF10B

GAPDH

C

AXIN1

CCND2

CTNNB1

FAH

HEIH

IGFBP3

MSH2

PDGFRL

RASSF1

SOCS3

TNFSF10

LDHA

D

BAX

CDH13

CXCR4

FAS

HEPN1

IRS1

MSH3

PEG10

RB1

STARD13

TP53

NONO

E

BCL2

CDKN1A

DAB2IP

FGL1

HGF

ITGB1

MTDH

PIN1

RELN

SULF1

URGCP

PPIH

F

BID

CDKN1B

DLC1

FLT1

HHIP

KDR

MTUS1

PSMD10

RHOA

TCF4

VEGFA

GDC

G

BIRC5

CDKN2A

E2F1

FZD7

HPSE

LEF1

MYC

PTEN

SFRP2

TGFA

XIAP

PPC

H

CASP8

CFLAR

EGF

GADD45B

HRAS

MAGEC2

NFKB1

PTGS2

SMAD4

TGFB1

ZDHHC2

NTC

 

* gene selection may be updated based on new research and development

货号GK042
产地美国
缩写GQH-HCC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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