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人巨噬细胞细胞生物学qPCR检测试剂盒

英文名:Human Macrophage Cell Biology qPCR Array Kit

货号:GK037

价格:$250

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ScienCell's GeneQuery™ Human Macrophage Cell Biology qPCR Array Kit (GQH-MAC) is designed to facilitate gene expression profiling of 88 key genes involved in macrophage activation and function.  Macrophages are an important component of the human immune response system and are activated for various purposes.  Brief examples of how included genes may be grouped according to activation or function are shown below:

  • Inflammatory response: F2R, PTAFR, NPY1R, PTGER1, TACR1
  • Chemotaxis: CXCL8, MMP2, MMP9, PTGS2, FPR1
  • Classical activation: CCL15, IL12A, NOS2, TNF, IFNG
  • Regulatory activation: IL10, CD80, CD86, SPHK1, CCL1
  • Wound healing activation: ARG1, LPAR1, SIPR1, IL27RA, MRC1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Macrophage Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic) 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADGRE1

CCL15

CD80

CXCL8

F2RL2

IFNB1

IL27RA

LTB4R2

NOX1

PTGER4

SOCS1

β-actin

B

ADGRE2

CCL17

CD86

CXCL9

FCER2

IFNG

IL4R

MAPK1

NPY1R

PTGES2

SPHK1

GAPDH

C

ADGRE5

CCL18

CD93

CXCR4

FCGR1A

IGF1

IL6

MARCO

NPY2R

PTGS2

SRA1

LDHA

D

ARG1

CCL20

CIITA

EDNRA

FCGR1B

IL10

ITGAM

MMP2

PIK3R1

SIGLEC1

STAB1

NONO

E

ARG2

CCL22

CLEC4A

EDNRB

FCGR3A

IL12A

LAMP1

MMP9

PTAFR

SIPR1

TACR1

PPIH

F

C5AR1

CCR5

CLEC7A

F13A1

FPR1

IL12B

LPAR1

MRC1

PTGER1

SIPR2

TGM2

GDC

G

C5AR2

CD163

CXCL10

F2R

FPR3

IL1A

LPAR2

NFKB1

PTGER2

SIPR5

TNF

PPC

H

CCL1

CD68

CXCL11

F2RL1

IDO1

IL23A

LTB4R

NOS2

PTGER3

SLAMF8

TNFRSF18

NTC\

 *gene selection may be updated based on new research and development

货号GK037
产地美国
缩写GQH-MAC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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