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人类HIF1信号响应qPCR检测试剂盒

英文名:Human HIF1 Signaling Response qPCR Array Kit

货号:GK036

价格:$250

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ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation.  HIF1 activity is a major component of the human response to hypoxia.  Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:

  • Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2
  • Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1
  • Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF
  • Metabolism: PKM, STC2, DHX35, DDX11, ERO1A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCB1

BHLHE40

COL18A1

DUSP6

HMOX1

LOXL2

NEK4

PKM

PRC1

RRAS

TSFM

β-actin

B

ADORA2A

BRCA1

COL1A1

ELK1

IGFBP3

MAP3K5

NOTCH4

PLCG2

PSME3

RRM2

TXNIP

GAPDH

C

ADORA2B

CCNE1

COL1A2

ERO1A

INHBA

MCM4

P4HA1

PLOD1

PTGS1

SKP2

UBE2S

LDHA

D

AK3

CCNE2

COL5A1

EXOSC2

INHBE

MEF2A

P4HA2

PLOD2

PTPRB

SMAD9

VEGFA

NONO

E

ANGPTL4

CCNF

CXCR4

FOS

INSR

MLLT10

PAM

POLE2

PTPRR

STC1

VEGFC

PPIH

F

BARD1

CDC6

DDX11

GADD45B

KDM3A

MMP2

PDZD2

POLR3K

RB1

STC2

VLDLR

GDC

G

BCL2

CDK1

DHFR

GINS2

LEPR

MXI1

PELI2

PPARD

RFC5

TFAM

ZNF292

PPC

H

BCL6

CDKN1C

DHX35

HES1

LOX

NDRG1

PGF

PPARG

RNH1

TRIP13

ZWINT

NTC

 

*gene selection may be updated based on new research and development

货号GK036
产地美国
缩写GQH-HFT
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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