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人体缺氧反应qPCR检测试剂盒

英文名:Human Hypoxia Response qPCR Array Kit

货号:GK035

价格:$250

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Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions.  The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region.  ScienCell's GeneQuery™ Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions.  Brief examples of how included genes may be grouped according to response type are shown below:

  • Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1
  • Inflammation: CXCL8, IL1A, CCL2, IL6, THBD
  • Vasorelaxation: AHSP, PTGIS
  • Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO
  • Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Endothelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AHSP

BNIP3L

EGLN1

EPO

HIF3A

JMJD6

OS9

PIK3CA

PTGIR

SAT2

TEK

β-actin

B

ALDOA

CA9

EGLN2

F2

HK1

KDR

PDGFB

PIM1

PTGIS

SELP

THBD

GAPDH

C

ANXA2

CCL2

EGLN3

FLT1

HRH1

LDHA

PDPK1

PLAU

PTGS2

SENP1

TNF

LDHA

D

APEX1

COPS5

EGR1

FOXO4

ICAM1

LGALS3

PECAM1

PPP3CB

RACK1

SLC2A1

TP53

NONO

E

ARNT

CREBBP

EIF4E

GSK3B

IFNG

LTC4S

PER1

PPP3CC

RPS6

SP1

TXN

PPIH

F

ARNT2

CUL2

ENO1

GYS1

IL1A

MAP3K1

PFKFB3

PRKCA

RUVBL2

STAT3

USP20

GDC

G

ARNTL

CXCL8

EP300

HIF1A

IL4

NFKB1

PFKFB4

PSMC6

RWDD3

TCEB1

VCAM1

PPC

H

BNIP3

EDN1

EPAS1

HIF1AN

IL6

NOS3

PGK1

PTGFR

SAT1

TCEB2

VHL

NTC

 

 * gene selection may be updated based on new research and development

 

货号GK035
产地美国
缩写GQH-HPX
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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