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人肺纤维化qPCR检测试剂盒

英文名:Human Pulmonary Fibrosis qPCR Array Kit

货号:GK034

价格:$250

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ScienCell's GeneQuery™ Human Pulmonary Fibrosis qPCR Array Kit (GQH-PFS) is designed to facilitate gene expression profiling of 88 key genes involved in pulmonary fibrosis. Pulmonary fibrosis is caused by excessive extracellular matrix (ECM) deposition during wound healing and tissue remodeling. The scarring of lung leads to thickened, stiff tissues and respiratory problems over time. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Pulmonary surfactant proteins: SFTPA2, SFTPAC
  • Inflammatory chemokines: CCL2, CCL3, CCL11, CCR2, CCR3, CXCL8
  • Inflammatory cytokines: PDGFA, CSF2, IL5, IL6, IL13
  • ECM remodeling: LOX, MMP1, MMP2, MMP8, PLAT, PLAU, PLG, SERPINA1, SERPINE2, TIMP1, TIMP2, TIMP3
  • Growth factors: EGF, IGF1, HGF, FGF1, FGF2, AGT, EDN1, CTGF
  • TGFB signaling pathway: SKIL, SPP1, TGFB1, TGFB2

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Pulmonary Fibrosis qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CCL3

CXCL8

FAM13A

HPS3

IL13

MMP1

NFE2L2

PLAU

SFTPD

TGFA

β-actin

B

AGT

CCL5

CYSLTR2

FGF1

HPS4

IL13RA2

MMP13

NFKB1

PLG

SKIL

TGFB1

GAPDH

C

ATP11A

CCR2

DSP

FGF2

HPS5

IL1B

MMP2

NFKB2

PTX3

SMAD3

TGFB2

LDHA

D

BMP7

CCR3

EDN1

FGF7

HPS6

IL5

MMP3

OBFC1

SERPINA1

SMAD7

TIMP1

NONO

E

CALCA

CEBPB

EGF

GREM1

IFNG

IL6

MMP8

PARN

SERPINE1

SNAI1

TIMP2

PPIH

F

CCL11

CSF2

ELMOD2

HGF

IGF1

LOX

MMP9

PDGFA

SERPINH1

SPP1

TIMP3

GDC

G

CCL18

CSF3

ELN

HMOX1

IL10

LPAR1

MT2A

PDGFB

SFTPA2

TERC

TIMP4

PPC

H

CCL2

CTGF

FAM111B

HPS1

IL12B

MECP2

MUC5B

PLAT

SFTPC

TERT

TNF

NTC

 

* gene selection may be updated based on new research and development

货号GK034
产地美国
缩写GQH-PFS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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