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人类非小细胞肺癌qPCR检测试剂盒

英文名:Human Non-Small Cell Lung Cancer qPCR Array Kit

货号:GK033

价格:$250

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ScienCell's GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Kit (GQH-NLC) is designed to facilitate gene expression profiling of 88 key genes involved in non-small cell lung cancer (NSCLC) carcinogenesis. NSCLC accounts for more than 80% of all lung cancers. There are three main types of NSCLC: adenocarcinoma, lung squamous cell carcinoma and lung large cell carcinoma. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Oncogenes and tumor suppressor genes: KRAS, HRAS, EML4, ALK, RARB, FHIT, RASSF1, TP53, CDKN2A
  • Cell cycle regulation and apoptosis: CDK4, CDK6, CCND1, CCNE2, CASP3, CASP9, BAD, STK4, FOXO3
  • MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
  • PI3K/AKT pathway: PI3Ks, AKT1, AKT2
  • Genes related to specific lung cancer types
    • Adenocarcinoma: BRAF, ERBB2, TGFBR2, SMAD4, EPCAM, BIRC5
    • Lung squamous cell carcinoma: FGFR2, CYPIA1, PTHLH, SOX2, PEBP1
    • Lung large cell carcinoma: ROS1, NME1, TTN, MYO18B, EPHA5, WNK3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTR3B

CALB2

CDK4

EPHA3

GRAMD4

MAP2K2

NRAS

PIK3R1

RAF1

RXRA

SOX2

β-actin

B

AKT1

CASK

CDK6

EPHA5

GRB2

MAPK1

NTRK3

PIK3R3

RAP2B

RXRG

STK11

GAPDH

C

AKT2

CASP3

CDKN2A

ERBB2

HRAS

MAPK3

OGG1

PIK3R5

RARB

S100A2

STK4

LDHA

D

ALK

CASP9

CYP1A1

FAM83B

KRAS

MMP2

PDPK1

PLCG2

RASSF1

S100A7

TGFA

NONO

E

ARAF

CCND1

DDR2

FGFR1

KRT19

MUC1

PEBP1

PRKCG

RASSF5

SFTPB

TP53

PPIH

F

BAD

CDC42BPB

E2F2

FHIT

KRT20

MYO18B

PIK3CA

PRKCI

RB1

SFTPC

TTN

GDC

G

BRAF

CDH1

EGF

FIGF

KRT7

NKX2-1

PIK3CD

PTGS2

RBMS3

SOS1

VEGFA

PPC

H

CADM1

CDH10

EML4

FOXO3

MAP2K1

NME1

PIK3CG

PTHLH

ROS1

SOS2

WNK3

NTC

 

* gene selection may be updated based on new research and development

货号GK033
产地美国
缩写GQH-NLC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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