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人类小细胞肺癌qPCR检测试剂盒

英文名:Human Small Cell Lung Cancer qPCR Array Kit

货号:GK032

价格:$250

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ScienCell's GeneQuery™ Human Small Cell Lung Cancer qPCR Array Kit (GQH-SLC) is designed to facilitate gene expression profiling of 88 key genes involved in small cell lung cancer (SCLC) carcinogenesis. SCLC accounts for approximately 10-20% of all lung cancers. Compared to non-small cell lung cancer (NSCLC), SCLC usually grows and metastasizes more rapidly. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Oncogenes and tumor suppressor genes: MYC, RARB, FHIT, TP53, PTEN
  • Cell cycle regulation and apoptosis: CDK2, CDK4, CDK6, CCND1, CCNE2, E2F2, CASP9
  • PI3K/AKT pathway: PI3Ks, AKT1, AKT2
  • IKK/NFKB pathway: NFKB1, CHUK, IKBKB

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Small Cell Lung Cancer qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ABCB1

CCND1

CHUK

CSF3

HTATIP2

KRT19

LAMB2

MYCL

PIK3CA

PTK2

SYP

β-actin

B

ABCC1

CCNE2

CKS1B

CXCR2

IKBKB

KRT20

LAMB3

NCAM1

PIK3CD

RARB

TOP1

GAPDH

C

AKT1

CDK2

CKS2

CYCS

ITGA2

KRT7

LAMB4

NFKB1

PIK3CG

RASSF1

TP53

LDHA

D

AKT2

CDK4

COL4A1

E2F2

ITGA2B

LAMA1

LAMC1

NKX2-1

PIK3R1

RCVRN

TRAF1

NONO

E

APAF1

CDK6

COL4A2

EGFR

ITGA3

LAMA2

LAMC2

NMB

PIK3R3

RXRA

TRAF4

PPIH

F

BIRC2

CDKN1B

COL4A4

ENO2

ITGAV

LAMA3

LAMC3

NMBR

PIK3R5

RXRG

TRAF5

GDC

G

BIRC7

CDKN2A

COL4A5

FHIT

ITGB1

LAMA5

MAX

NOS2

PTEN

SKP2

TRAF6

PPC

H

CASP9

CHGA

COL4A6

FN1

KIT

LAMB1

MYC

PIAS2

PTGS2

SST

XIAP

NTC

 

* gene selection may be updated based on new research and development

货号GK032
产地美国
缩写GQH-SLC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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