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Human Tie2 / Angpt信号qPCR检测试剂盒

英文名:Human Tie2/Angpt Signaling qPCR Array Kit

货号:GK029

价格:$250

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ScienCell's GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Kit (GQH-TEK) is designed to facilitate gene expression profiling of 88 key genes involved in Tie2 signaling transduction, which is a key component in the formation of blood vessels during angiogenesis.  Brief examples of how included genes may be grouped according to function are shown below:

  • Ligands & receptors: ANGPT1, ANGPT2, TEK, TIE1
  • Morphology: GRB2, PTPN11, SOS1, PTK2, RHOA  
  • Motility:  PLD1, MAP2K1, DOK2, NCK1, CRK
  • Transcription factors: STAT1, STAT5B, MEF2A, FOXO3, EGR1
  • Cell cycle regulation: CDC42, NFKB1, TNIP2, RIPK1, DIABLO

 

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Tie2/Angpt Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADSS

CDC42

DOK2

FOXO3

HIF1A

ITGB5

MMP2

PDIA3

PTPN11

SELE

TEK

β-actin

B

AKT1

CDKN1A

DOK4

FYN

HRAS

KLF2

MTOR

PGK1

PXN

SELP

TIAM1

GAPDH

C

AKT2

CRK

EGR1

GJA5

ICAM1

MAP2K1

NCK1

PIK3CA

RAC1

SOS1

TIE1

LDHA

D

ALDOA

CTNNB1

ELF2

GRB14

ILK

MAPK1

NFKB1

PIK3R1

RAF1

SPHK1

TLN1

NONO

E

ANGPT1

CXCL12

ELK1

GRB2

ITGA5

MAPK14

NOS3

PIK3R2

RASA1

STAT1

TNIP2

PPIH

F

ANGPT2

DIABLO

EPAS1

GRB7

ITGAV

MAPK3

NOX1

PLCG1

RELA

STAT3

TXNRD1

GDC

G

ARHGAP5

DIAPH1

FES

GYS1

ITGB1

MAPK7

NR4A1

PLD1

RHOA

STAT5A

VCAM1

PPC

H

BIRC5

DLL4

FOXO1

HAGH

ITGB3

MEF2A

PAK1

PTK2

RIPK1

STAT5B

VEGFA

NTC

  * gene selection may be updated based on new research and development

货号GK029
产地美国
缩写GQH-TEK
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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