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人类Wnt信号通路qPCR检测试剂盒

英文名:Human Wnt Signaling Pathway qPCR Array Kit

货号:GK026

价格:$250

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ScienCell's GeneQuery™ Human Wnt Signaling Pathway qPCR Array Kit (GQH-WNT) is designed to facilitate gene expression profiling of 88 key genes involved in Wnt signaling transduction.  The Wnt signaling pathway is well known for its role in carcinogenesis and embryonic development.  This kit focuses on canonical Wnt signaling.  Brief examples of how included genes may be grouped according to purpose are shown below:

  • Ligands: all known WNTs
  • Receptors: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6
  • Receptor transducers:  DVL1, DVL2, DVL3, CSNK1E, MARK1
  • Transcription factors: CTNNB1, TCF7L1, TCF7L2, LEF1, PYGO1
  • Regulatory elements: NLK, NKD1, AES, SFRP1, LRP1

 

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Wnt Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AES

CSNK2A1

DKKL1

FZD2

GSK3A

LRP1

PORCN

RSPO4

SMO

WNT16

WNT6

β-actin

B

APC

CTNNB1

DVL1

FZD3

GSK3B

LRP5

PPP2R4

RYK

TCF7L1

WNT2

WNT7A

GAPDH

C

AXIN1

CTNNBIP1

DVL2

FZD4

HNF1A

LRP6

PYGO1

SFRP1

TCF7L2

WNT2B

WNT7B

LDHA

D

AXIN2

CUL1

DVL3

FZD5

KREMEN1

MARK1

ROR1

SFRP2

TLE1

WNT3

WNT8A

NONO

E

BCL9

DKK1

FBXW7

FZD6

LEF1

MARK2

ROR2

SFRP4

WNT1

WNT3A

WNT8B

PPIH

F

CDC37

DKK2

FRZB

FZD7

LGR4

MARK3

RSPO1

SKP1

WNT10A

WNT4

WNT9A

GDC

G

CSNK1A1L

DKK3

FZD1

FZD8

LGR5

NKD1

RSPO2

SKP2

WNT10B

WNT5A

WNT9B

PPC

H

CSNK1E

DKK4

FZD10

FZD9

LGR6

NLK

RSPO3

SMARCA4

WNT11

WNT5B

ZNRF3

NTC

 

 * gene selection may be updated based on new research and development

货号GK026
产地美国
缩写GQH-WNT
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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