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人类Notch信号通路qPCR检测试剂盒

英文名:uman Notch Signaling Pathway qPCR Array Kit

货号:GK025

价格:$250

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ScienCell's GeneQuery™ Human Notch Signaling Pathway qPCR array kit (GQH-NCH) is designed to facilitate gene expression profiling of 88 key genes involved in the human Notch molecular pathway with an emphasis on Notch1 signal transduction.  Brief examples of how included genes may be grouped according to their roles are shown below:

 

  • Canonical ligands: JAG1, JAG2, DLL1, DLL3DLL4
  • Noncanonical ligands: WNT1, DNER, CNTN1, NOV, SHH
  • Receptors: NOTCH1, NOTCH2, NOTCH3, NOTCH4
  • Transcriptional repressors: HEY1, HEY2, ESR1, HES1, HES7
  • Proteolysis: ADAM17, PSEN1, FURIN, POFUT1, PSENEN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Notch Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACBD3

CDKN1A

DLL1

DVL2

GSK3B

IL2RA

MAML3

NCOR2

NRARP

PSENEN

SMAD2

β-actin

B

ADAM10

CIR1

DLL3

EGFL7

HES1

JAG1

MEF2C

NEURL1

NUMB

PTF1A

SMO

GAPDH

C

ADAM17

CLTA

DLL4

ESR1

HES5

JAG2

MFAP2

NFKB2

NUMBL

RBPJ

SNW1

LDHA

D

APH1A

CNTN1

DNAAF3

ESR2

HES7

JUN

MFAP5

NOTCH1

PCSK5

RFNG

SPEN

NONO

E

AXIN1

CNTN6

DNER

FBXW7

HEY1

LFNG

MFNG

NOTCH2

POFUT1

RITA1

TBP

PPIH

F

CCND1

CTNNB1

DNM1

FHL1

HEY2

MAGI2

MIB1

NOTCH3

POGLUT1

RUNX1

TCF7L2

GDC

G

CDK2

DLK1

DTX1

FURIN

HEYL

MAML1

MIB2

NOTCH4

PSEN1

SHH

THBS2

PPC

H

CDK8

DLK2

DTX4

GATA3

HIF1A

MAML2

MLLT4

NOV

PSEN2

SIRT1

WNT1

NTC

 

 

* gene selection may be updated based on new research and development

货号GK025
产地美国
缩写GQH-NCH
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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