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人内皮细胞分化qPCR检测试剂盒

英文名:Human Endothelial Cell Differentiation qPCR Array Kit

货号:GK023

价格:$250

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ScienCell's GeneQuery™ Human Endothelial Cell Differentiation qPCR Array Kit (GQH-ECD) is designed to facilitate gene expression profiling of 88 key genes to help characterize endothelial cells during lineage differentiation and is based on extensive literature research.  Brief examples of how included genes may be grouped according to specification markers are shown below:

  • Circulating early endothelial progenitors: MAFB, RB1, ADORA3, MYCL1, NCOA2
    • Circulating late endothelial progenitors: FOXF1, ITGAM, SIX4, EGFR, CREB5
    • Hemangioblasts: GATA1, LMO2, PROM1, RUNX1, TAL1
    • Lymphatic endothelial cells: FLT4, LYVE1, PDPN, PROX1, FGFR3
    • EndMT: ACTA2, SMAD3, S100A4, TWIST, BMP7

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

 

 GeneQuery™ Human Endothelial Cell Differentiation qPCR Array Plate Layout *

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACE

CD34

EGFR

FOXF1

IL1R1

LYVE1

NFYA

PODXL

RUNX1

SLC2A9

TAL1

ACTB

B

ACTA2

CD36

EPAS1

GATA1

ITGAM

MAFB

NODAL

PROM1

S100A4

SMAD1

TBX18

GAPDH

C

ADORA3

CDH1

EFNB2

GATA6

JAG1

MAP3K10

NOTCH1

PROX1

SEMA6D

SMAD3

TEK

LDHA

D

BMP10

CDH5

EPHB4

GDF2

JDP2

MAPK14

NR2F2

PTGFR

SHH

SMAD5

TGFBR3

NONO

E

BMP4

CFC1

ETV1

GSC

KDR

MCAM

NRP1

RB1

SIGLEC1

SNAI1

TRPS1

PPIH

F

BMP7

CREB5

FGFR3

GSK3B

LILRB5

MYCL

NRP2

RHOA

SIX4

SOX18

TWIST1

GDC

G

CD163

CTNNB1

FLT1

HDAC5

LMO2

NCOA2

PDPN

ROCK1

SLAMF8

T

VCAM1

PPC

H

CD248

CXADR

FLT4

ICAM2

LPAR1

NFATC1

PECAM1

ROR2

SLC16A3

TAGLN

ZEB1

NTC

 

* gene selection may be updated based on new research and development

货号GK023
产地美国
缩写GQH-ECD
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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