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人类T细胞受体信号通路qPCR检测试剂盒

英文名:Human T Cell Receptor Signaling Pathway qPCR Array Kit

货号:GK021

价格:$250

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ScienCell's GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • T cell receptor complex components: CD3D, CD3E, CD3G
  • Co-receptors: CD4, CD8A, CD8B
  • Co-inhibitors: PDCD1, CTLA4
  • Co-stimulators: ICOS, CD28
  • Kinases: FYN, ZAP70
  • MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
  • PI3K/AKT pathway: PI3Ks, AKT1, AKT2
  • IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CD274

CD8A

DYRK1A

HRAS

MALT1

NCK1

NFKBIE

PDPK1

PPP3CB

RELA

β-actin

B

AKT2

CD28

CD8B

DYRK2

ICOS

MAP2K1

NCK2

NRAS

PIK3CA

PPP3R1

SOS1

GAPDH

C

BCL10

CD3D

CDC42

FOS

IKBKB

MAP2K7

NFATC1

NRON

PIK3CB

PRKCA

SOS2

LDHA

D

CALM1

CD3E

CDK4

FYN

IKBKG

MAP3K7

NFATC2

PAK1

PIK3R1

PRKCQ

TEC

NONO

E

CARD11

CD3G

CHUK

GRAP2

ITK

MAP3K8

NFATC3

PAK2

PIK3R2

PTCRA

TNF

PPIH

F

CBL

CD4

CTLA4

GRB2

JUN

MAPK1

NFKB1

PAK3

PIK3R3

PTPN6

VAV1

GDC

G

CBLB

CD80

DAG1

GSK3A

KRAS

MAPK3

NFKBIA

PAK4

PLCG1

RAF1

VAV2

PPC

H

CBLC

CD86

DLG1

GSK3B

LCP2

MAPK9

NFKBIB

PDCD1

PPP3CA

RASGRP1

ZAP70

NTC

 

* gene selection may be updated based on new research and development

货号GK021
产地美国
缩写GQH-TRS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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