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人B细胞受体信号通路qPCR检测试剂盒

英文名:Human B Cell Receptor Signaling Pathway qPCR Array Kit

货号:GK020

价格:$250

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ScienCell's GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • B cell receptor complex components: CD79A, CD79B
  • Co-inhibitory receptors: CD22, CD72, FCGR2B
  • Co-stimulators: CD81, CR2, IFITM1
  • Expression adaptor proteins: BLNK, GAB2, GRB2, CARD11
  • Kinases: LYN, SYK, PI3Ks
  • Phosphatases: INPP5D, PTPN6
  • MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
  • PI3K/AKT pathway: PI3Ks, AKT1, AKT2
  • IKK/NFKB pathway: NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

AKT1

CD22

DAPP1

GSK3B

INPP5D

MAP2K1

MYC

NFKBIE

PIK3R3

PRKCD

RELA

β-actin

B

AKT2

CD72

ELK1

GTF2I

INPPL1

MAP3K7

NCK1

NRAS

PIP5K1A

PTPN18

SH3BP2

GAPDH

C

ATF2

CD79A

FCGR2B

HCLS1

IRF4

MAP4K1

NFATC1

PDPK1

PIP5K1C

PTPN6

SHC1

LDHA

D

BCL10

CD79B

FOS

HRAS

JUN

MAPK1

NFATC2

PIK3AP1

PLCG2

RAC1

SOS1

NONO

E

BLNK

CD81

FYN

IFITM1

KRAS

MAPK3

NFATC3

PIK3CA

PPP3CA

RAC2

SOS2

PPIH

F

BTK

CHUK

GAB2

IKBKB

LAT2

MAPK4

NFKB1

PIK3CB

PPP3CB

RAF1

SYK

GDC

G

CARD11

CR2

GRB2

IKBKG

LYN

MAPK9

NFKBIA

PIK3R1

PPP3R1

RASGRP3

TEC

PPC

H

CBL

DAG1

GSK3A

ILF2

MALT1

MEF2D

NFKBIB

PIK3R2

PRKCB

REL

VAV2

NTC

 * gene selection may be updated based on new research and development

货号GK020
产地美国
缩写GQH-BRS
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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