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人血管生成qPCR检测试剂盒

英文名:Human Angiogenesis qPCR Array Kit

货号:GK017

价格:$250

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ScienCell's GeneQuery™ Human Angiogenesis qPCR array kit (GQH-ANG) is designed to facilitate gene expression profiling of 88 key genes involved in angiogenesis during differing environmental conditions based on extensive literature research.  Brief examples of how included genes may be grouped according to function or condition are shown below:

 

  • by stages of angiogenesis:
  • Sprouting: LGALS9, DLL4, NOTCH1, FLT1, KDR
  • Migration: FGF1, PECAM1, CD82, ITGB1, NRP1
  • ECM remodeling: MMP2, MMP9, ITGAV, LAMA5, NRP1
  • Maturation: ANGPT1, VCAM1, PDGFRB, FOS, SELE
  • Permeability: CDH2, CDH5, ANGPT2, EDN1, KLF2

 

  • by environmental conditions:
  • Hypoxia: HIF1A, NOS3, EGLN1, PDGFRA, ITGA5
  • Inflammation: EPHA2, IL1B, PTGS2, TNFAIP1, CCL2
  • Wound healing: FGF2, ID1, ITGB3, COL4A3, PLAU
  • Coagulation: CNTN1, CD36, SERPINE1, FN1, THBS1
  • Signal regulation: EFNB2, BMP1, MEOX2, HHEX, VEGFA

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Angiogenesis qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note: all gene names follow their official symbols by HGNC

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ANGPT1

CDH2

DVL3

EREG

FOXO1

ICAM1

JAG1

MMP9

PDGFRB

RUNX2

TEK

ACTB

B

ANGPT2

CDH5

EDN1

FGF1

FZD4

ID1

KDR

NOS3

PECAM1

SELE

TGFB1

GAPDH

C

BAIAP3

CNTN1

EDNRA

FGF2

HAND2

IL1B

KIT

NOTCH1

PF4

SEMA3F

TGFB2

LDHA

D

BMP1

COL4A3

EFNB2

FIGF

HEY2

IL6

KLF2

NOTCH4

PGF

SERPINE1

THBS1

NONO

E

CCL2

CX3CL1

EGLN1

FLT1

HHEX

ITGA5

LAMA5

NRP1

PLAU

SERPINF1

TNFAIP1

PPIH

F

CCL5

CXCR4

ENG

FN1

HIF1A

ITGAV

LGALS9

NRP2

PODXL

SMAD2

VCAM1

GDC

G

CD36

DICER1

EPHA2

FOS

HMOX1

ITGB1

MAGI1

PARD3

PROK2

SMAD3

VEGFA

PPC

H

CD82

DLL4

ERBB2

FOXC2

HOXB3

ITGB3

MMP2

PDGFRA

PTGS2

SPHK1

VEGFC

NTC

 

* gene selection may be updated based on new research and development

货号GK017
产地美国
缩写GQH-ANG
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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