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人类基底细胞癌qPCR检测试剂盒

英文名:Human Basal Cell Carcinoma qPCR Array Kit

货号:GK015

价格:$250

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ScienCell's GeneQuery™ Human Basal Cell Carcinoma qPCR Array Kit (GQH-BCC) is designed to facilitate gene expression profiling of key genes involved in (i) basal cell carcinoma (BCC) biology and carcinogenesis; (ii) targeted BCC therapies; and (iii) hedgehog signaling pathway. 88 genes are selected in this kit based on database and literature research.      

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ qPCR Array Kit Controls

Each GeneQuery™ plate contains eight controls (Figure 1).

  • Five target housekeeping genes (β-actin, GAPDH, LDHA, NONO, and PPIH), which enable normalization of data.
  • The Genomic DNA (gDNA) Control (GDC) detects possible gDNA contamination in the cDNA samples. It contains a primer set targeting a non-transcribed region of the genome.
  • Positive PCR Control (PPC) tests whether samples contain inhibitors or other factors that may negatively affect gene expression results. The PPC consists of a predispensed synthetic DNA template and a primer set that can amplify it. The sequence of the DNA template is not present in the human genome, and thus tests the efficiency of the polymerase chain reaction itself.
  • The No Template Control (NTC) is strongly recommended, and can be used to monitor the DNA contamination introduced during the workflow such as reagents, tips, and the lab bench.

GeneQuery™ Human Basal Cell Carcinoma qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

APC

CD151

CSNK1G2

EIF4E

FZD5

GSK3B

LRP2

RAB23

TCF7L1

WNT11

WNT5B

β-actin

B

APC2

CDH1

CSNK1G3

FAT2

FZD6

HHATL

MC1R

ROR2

TCF7L2

WNT16

WNT7A

GAPDH

C

AXIN1

CEBPA

CTNNB1

FBXW11

FZD7

HHIP

NET1

SHFM1

TGFBR2

WNT2

WNT7B

LDHA

D

AXIN2

CSNK1A1

DHH

FZD1

FZD8

IFNA1

PARP1

SHH

TP53

WNT2B

WNT8A

NONO

E

BMP2

CSNK1A1L

DVL1

FZD10

FZD9

IHH

PRKACA

SMO

TXN

WNT3

WNT8B

PPIH

F

BMP4

CSNK1D

DVL2

FZD2

GAS1

KLF4

PRKACB

STAT2

WNT1

WNT3A

WNT9A

GDC

G

BTRC

CSNK1E

DVL3

FZD3

GLI2

LEF1

PRKACG

SUFU

WNT10A

WNT4

WNT9B

PPC

H

CASP3

CSNK1G1

EGF

FZD4

GLI3

LOC400927

PTCH1

TCF7

WNT10B

WNT5A

ZIC2

NTC

 

* gene selection may be updated based on new research and development

货号GK015
产地美国
缩写GQH-BCC
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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