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人类黑素细胞发育和色素沉着qPCR检测试剂盒

英文名:Human Melanocyte Development & Pigmentation qPCR Array Kit

货号:GK010

价格:$250

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ScienCell's GeneQuery™ human melanocyte development & pigmentation qPCR array kit (GQH-MDP) is designed to facilitate gene expression profiling of key genes involved in melanocyte development and pigmentation pathways. 40 genes are selected in this kit based on database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery™ Human Melanocyte Development & Pigmentation qPCR Array Plate Layout* (8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY3

CTNNB1

GRB2

MC1R

PRKACA

β-actin

ADCY3

CTNNB1

GRB2

MC1R

PRKACA

β-actin

B

AKT1

EDNRB

GSK3B

MITF

QPCT

GAPDH

AKT1

EDNRB

GSK3B

MITF

QPCT

GAPDH

C

AKT3

EP300

HRAS

MLANA

RPS6KA1

LDHA

AKT3

EP300

HRAS

MLANA

RPS6KA1

LDHA

D

BCL2

FOXD3

KIT

OCA2

SOS1

NONO

BCL2

FOXD3

KIT

OCA2

SOS1

NONO

E

BRAF

FZD3

KITLG

PAX3

SOX10

PPIH

BRAF

FZD3

KITLG

PAX3

SOX10

PPIH

F

CDH1

FZD4

LEF1

PIK3CA

TRP2

GDC

CDH1

FZD4

LEF1

PIK3CA

TRP2

GDC

G

CREB1

GNB2L1

MAPK1

PMEL

TYR

PPC

CREB1

GNB2L1

MAPK1

PMEL

TYR

PPC

H

CREBBP

GPR143

MAPK3

POMC

TYRP1

NTC

CREBBP

GPR143

MAPK3

POMC

TYRP1

NTC

 

* gene selection may be updated based on new research and development

货号GK010
产地美国
缩写GQH-MDP
规格One 96-well plate, one 20ul reaction per well
用途科研
储存4°C
运输胶冰
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