ScienCell's GeneQuery™ human melanocyte development & pigmentation qPCR array kit (GQH-MDP) is designed to facilitate gene expression profiling of key genes involved in melanocyte development and pigmentation pathways. 40 genes are selected in this kit based on database and literature research.
GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Melanocyte Development & Pigmentation qPCR Array Plate Layout* (8 controls in Bold and Italic)
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1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ADCY3 |
CTNNB1 |
GRB2 |
MC1R |
PRKACA |
β-actin |
ADCY3 |
CTNNB1 |
GRB2 |
MC1R |
PRKACA |
β-actin |
B |
AKT1 |
EDNRB |
GSK3B |
MITF |
QPCT |
GAPDH |
AKT1 |
EDNRB |
GSK3B |
MITF |
QPCT |
GAPDH |
C |
AKT3 |
EP300 |
HRAS |
MLANA |
RPS6KA1 |
LDHA |
AKT3 |
EP300 |
HRAS |
MLANA |
RPS6KA1 |
LDHA |
D |
BCL2 |
FOXD3 |
KIT |
OCA2 |
SOS1 |
NONO |
BCL2 |
FOXD3 |
KIT |
OCA2 |
SOS1 |
NONO |
E |
BRAF |
FZD3 |
KITLG |
PAX3 |
SOX10 |
PPIH |
BRAF |
FZD3 |
KITLG |
PAX3 |
SOX10 |
PPIH |
F |
CDH1 |
FZD4 |
LEF1 |
PIK3CA |
TRP2 |
GDC |
CDH1 |
FZD4 |
LEF1 |
PIK3CA |
TRP2 |
GDC |
G |
CREB1 |
GNB2L1 |
MAPK1 |
PMEL |
TYR |
PPC |
CREB1 |
GNB2L1 |
MAPK1 |
PMEL |
TYR |
PPC |
H |
CREBBP |
GPR143 |
MAPK3 |
POMC |
TYRP1 |
NTC |
CREBBP |
GPR143 |
MAPK3 |
POMC |
TYRP1 |
NTC |
* gene selection may be updated based on new research and development
货号 | GK010 |
产地 | 美国 |
缩写 | GQH-MDP |
规格 | One 96-well plate, one 20ul reaction per well |
用途 | 科研 |
储存 | 4°C |
运输 | 胶冰 |