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人肝星状细胞生物学qPCR检测试剂盒
Human Hepatic Stellate Cell Biology qPCR Array Kit
品牌:
ScienCell
货号:
GK091
价格:
$286
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ScienCell's GeneQuery? Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:     

  • Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
  • Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
  • Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
  • Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
  • ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
  • Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
  • Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery? Human Hepatic Stellate Cell Biology qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

COL2A1

CXCL2

FGF1

IGF1

LAMB1

MYH10

NGFR

RARA

SPARC

TIMP1

β-actin

B

B9D1

COL4A2

CXCL3

FGF2

IGF2

LAMB3

MYH11

PDGFA

RBP1

SPP1

TMEM67

GAPDH

C

B9D2

CRABP1

CXCL8

FOXF1

IL1A

LAMC1

MYH9

PDGFB

RBP2

STX2

TNC

LDHA

D

BDNF

CRABP2

CYP26A1

GFAP

ITGB3

LEP

NCAM1

PDGFC

RBP4

SYNM

TNF

NONO

E

CC2D2A

CSF1

CYP26B1

HAS1

KLF6

LHX2

NCAM2

PDGFD

RBP5

SYP

VCAM1

PPIH

F

CCL2

CTGF

DES

HGF

LAMA1

LRAT

NES

PKD1

RELN

TGFB1

VCAN

GDC

G

CDH1

CXCL10

EGF

ICAM1

LAMA2

MMP13

NFKB1

PKHD1

RPGRIP1L

TGFBI

VIM

PPC

H

CLEC3B

CXCL12

F2

IFNG

LAMA3

MMP2

NFKB2

PTN

SLC17A5

THBS2

VTN

NTC

 

* gene selection may be updated based on new research and development

货号GK091
产地美国
缩写GQH-HSB
规格96Tests
用途科研
储存4度
运输胶冰
No Publication available at this time
There are no product questions yet. 
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